Estudio Teórico de la Función del Complejo Receptor-IP3 / Canales BK en Neurona Única
DOI:
https://doi.org/10.17488/RMIB.42.2.2Palabras clave:
Canales BK, Receptor IP3, Microdominios de Ca2, Simulaciones en neurona única, Bomba SERCAResumen
Los canales de potasio activados por calcio de larga conductancia (canales BK) cumplen múltiples funciones en el sistema nervioso central. La apertura de los canales BK requiere de un incremento de la concentración de calcio citosólico ([Ca2+]cyt) de dos maneras: entrada de Ca2+ por canales de calcio gatillados por voltaje (CaV) localizados en la membrana plasmática y por eflujo de calcio del retículo endoplásmico (ER) causado por 1,4,5-Trifosfato (IP3) o rianodina (RyR). La interacción BK/IP3/RyR ha sido ampliamente estudiada en músculo liso. Sin embargo, en neuronas ha sido poco estudiada y la función de esta interacción es poco clara. En este trabajo se presenta un modelo computacional de neurona con canales de Na+, K+ y BK y su interacción con [Ca2+]cyt proveniente del receptor a IP3 (IP3-R) del retículo endoplásmico. Está basado en el formalismo de Hodgkin-Huxley y el modelo de Goldbeter. Los modelos matemáticos fueron implementados en Visual Basic® y las ecuaciones diferenciales fueron resueltas numéricamente. Se exploraron varias condiciones de conductancia BK y eflujo de Ca2+ endoplásmico. Los resultados muestran que un incremento abrupto de [Ca2+]cyt (≥ 5 mM) activa los canales BK y producen una pausa o para del tren de potenciales de acción.
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Derechos de autor 2021 María Eugenia Pérez Bonilla, Marleni Reyes Monreal, Jessica Quintero Pérez, Miguel Pérez Escalera, Arturo Reyes Lazalde
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