Evaluation of Parameters in PLA and PCL Scaffolds to be Used in Cartilaginous Tissues

Authors

  • Klear Cea Universidad Austral de Chile, Chile
  • Marcelo Donoso Universidad Austral de Chile, Chile
  • Guillaume Sérandour Universidad Austral de Chile, Chile https://orcid.org/0000-0002-1211-1256
  • Gabriela Martínez Universidad Austral de Chile, Chile https://orcid.org/0000-0003-0515-9400
  • Luz Alegría Universidad Austral de Chile, Chile

DOI:

https://doi.org/10.17488/RMIB.42.2.12

Keywords:

Scaffold, Cartilaginous Tissue, 3D Printing

Abstract

The scopes of medical treatments involving organ transplants and implants for chronic problems and trauma have changed significantly. However, these procedures are subject to multiple problems. Recently, tissue engineering has been used to address them. The present study is framed in the field of tissue engineering, particularly cartilage tissue, and proposes the evaluation of geometric and impression parameters for the manufacture of scaffolds as a basis for the growth of cells through 3D impression techniques. These scaffolds are highly porous three-dimensional supports that house donated or himself patient cells, providing a surface where the cells can adhere and proliferate. In the methodology, geometric and pore size variables are defined for scaffolding modeling by using CAD techniques and standardization of the printing process with standard 3D printers and accessible materials. The results showed that material flow, printing temperature, printing speed and ventilation are the most influential parameters in the manufacture of scaffolds. Additionally, it was found micrometric variations between the modeled design and the printing result. These scaffolds will subsequently be subjected to in vitro cell culture evaluating the adherence, division, and proliferation of the cells.

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References

Serrato Ochoa D, Nieto Aguilar R, Aguilera Méndez A. Ingeniería de tejidos. Una nueva disciplina en medicina regenerativa. Investigación y Ciencia [Internet]. 2015;23(64):61-9. Available from: https://www.redalyc.org/articulo.oa?id=67441039009

Rosa V, Zhang Z, Grande RH, Nör JE. Dental Pulp Tissue Engineering in Full-length Human Root Canals. J Dent Res. 2013;92(11):970-5. Available from: https://doi.org/10.1177/0022034513505772

Shapira A, Kim D-H, Dvir T. Advanced micro- and nanofabrication technologies for tissue engineering. Biofabrication. 2014;6(2):020301. Available from: https://doi.org/10.1088/1758-5082/6/2/020301

Luquetti DV, Leoncini E, Mastroiacovo P. Microtia-anotia: A global review of prevalence rates. Birth Defects Res [Internet]. 2011;91(9):813–22. Available from: https://doi.org/10.1002/bdra.20836

Ingber DE, Mow VC, Butler D, Niklason L, et al. Tissue Engineering and Developmental Biology: Going Biometric. Tissue Eng [Internet]. 2006;12(12):3265-83. Available from: https://doi.org/10.1089/ten.2006.12.3265

Derby B. Printing and Prototyping of Tissues and Scaffolds. Science [Internet]. 2012;338(6109):921-6. Available from: https://doi.org/10.1126/science.1226340

Azevedo Gonçalves Mota RC, Oliveira da Silva E, Fortes de Lima F, Rodrigues de Menezes L, et al. 3D Printed Scaffolds as a New Perspective for Bone Tissue Regeneration: Literature Review. Mat Sci and Appl [Internet]. 2016;7(8):430-52. Available from: https://doi.org/10.4236/msa.2016.78039

Reboledo-Grau D, Martínez-Bordes G. Metodología para el diseño computacional de andamios a ser utilizados en reparación ósea. Rev UIS Ing [Internet]. 2020;19(4):301-14. Available from: https://doi.org/10.18273/revuin.v19n4-2020025

Boschetti PJ, Pelliccioni O, Da Costa K, Sabino MA. Lattice Boltzmann simulation of swelling of an implant for microtia manufactured with IPN hydrogel. Comput Methods Biomech Biomed Engin [Internet]. 2020;23(1):491-9. Available from: https://doi.org/10.1080/10255842.2020.1740210

Banjanin B, Vladic G, Pal M, Balos S, et al. Consistency analysis of mechanical properties of elements produced by FDM additive manufacturing technology. Rev Mater [Internet]. 2018;23(4):e12250. Available from: https://doi.org/10.1590/s1517-707620180004.0584

Chiesa-Estomba CM, Aiastui A, González-Fernández I, Hernáez-Moya R, et al. Three-Dimensional Bioprinting Scaffolding for Nasal Cartilage Defects: A Systematic Review. Tissue Eng Regen Med [Internet]. 2021;18(3):343-53. Available from: https://doi.org/10.1007/s13770-021-00331-6

Nava MM, Draghi L, Giordano C, Pietrabissa R. The Effect of Scaffold Pore Size in Cartilage Tissue Engineering. J Appl Biomater Funct Mater [Internet]. 2016;14(3):223-9. Available from: https://doi.org/10.5301/jabfm.5000302

Moroni L, de Wijn JR, van Blitterswijk CA. 3D fiber-deposited scaffolds for tissue engineering: Influence of pores geometry and architecture on dynamic mechanical properties. Biomaterials [Internet]. 2006;27(7):974–85. Available from: https://doi.org/10.1016/j.biomaterials.2005.07.023

3DXTECH. Technical Data Sheet: ECOMAX® PLA 3D Printing Filament [Internet]; 2021. Available from: https://www.3dxtech.com/

Meck. Polylactides (PLA) [Internet]. Sigma-Aldrich; 2021. Available from: https://www.sigmaaldrich.com/MX/es/products/materials-science/biomedical-materials/hydrophilic-polymers-

Autodesk. Inventor [Internet]. Autodesk; 2019. Available from: https://www.autodesk.com/education/free-software/inventor-professional

Ultimaker. Cura 4.0.0 [Internet]. Ultimaker; 2019. Available from: https://ultimaker.com/software/ultimaker-cura

Hot-World GmbH & Co. KG. Repetier-Host v2.1.6 [Internet]. Repetier; 2019. Available from: https://www.repetier.com

Czech Metrology Institute. Gwyddion 2.53 [Internet]. Gwyddion; 2019. Available from: http://gwyddion.net/download.php#stable

Graziano MU, Graziano KU, Pinto FM, Bruna CQ, et al. Effectiveness of disinfection with alcohol 70% (w/v) of contaminated surfaces not previously cleaned. Rev Latino Am. Enfermagem [Internet]. 2013;21(2):618–23. Available from: https://doi.org/10.1590/s0104-11692013000200020

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Published

2021-08-25

How to Cite

Cea, K., Donoso, M., Sérandour, G., Martínez, G., & Alegría, L. (2021). Evaluation of Parameters in PLA and PCL Scaffolds to be Used in Cartilaginous Tissues. Revista Mexicana De Ingenieria Biomedica, 42(2), 149–159. https://doi.org/10.17488/RMIB.42.2.12

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Research Articles

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